| Isabella of Burgundy | |
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| Tenure | 1284–1291 |
| Spouse | Rudolph I of Germany Pierre IX de Chambly, Lord of Neaufles |
| Issue | |
| Jeanne de Chambly, Dame de Neauphle-le-Chateau | |
| House | House of Burgundy |
| Father | Hugh IV, Duke of Burgundy |
| Mother | Beatrice of Champagne |
| Born | 1270 |
| Died | August 1323 |
Isabella of Burgundy (1270 – August 1323), Lady of Vieux-Château, was the second and last Queen consort of Rudolph I of Germany.
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She was the second daughter of Hugh IV, Duke of Burgundy and his second wife Beatrice of Champagne.
Her maternal grandparents were Theobald I of Navarre, also Count of Champagne, and his third wife Margaret of Bourbon.
Margaret was a daughter of Archambaud VIII of Bourbon, Sire de Bourbon (1216–1242), and his first wife Guigone of Forez.
She was betrothed in 1272 to Charles of Flandres. He was born in 1266 to the later Robert III of Flanders and his first wife Blanche of Sicily. His maternal grandparents were Charles I of Sicily and Beatrice of Provence. Her betrothed died in 1277.
On 6 February 1284, Isabella married Rudolph I of Germany. The bride was fourteen years old and the groom almost sixty-six. She seems to have been younger than eight of his children from his previous marriage to Gertrude of Hohenburg. She was a year older than her stepdaughter Judith of Habsburg, his ninth child and wife of Wenceslaus II of Bohemia.
Their marriage remained childless. Rudolph died on 15 July 1291. He was succeeded as Duke of Austria by his co-ruling sons Albert I and Rudolph II.
She returned to the Court of Burgundy and was granted the title of Lady of Vieux-Château on 20 November 1294
She had a second marriage to Pierre IX de Chambly, Lord of Neaufles who died c. 1319.
Isabella and Pierre de Chambly had at least one daughter and through her further descendants:
| Preceded by Gertrude of Hohenburg |
German Queen 6 February 1284 – 15 July 1291 |
Succeeded by Imagina of Isenburg-Limburg |